Journal: Antimicrobial Agents and Chemotherapy
Article Title: Antibiofilm activity of manogepix, ibrexafungerp, amphotericin B, rezafungin, and caspofungin against Candida spp. biofilms of reference and clinical strains
doi: 10.1128/aac.00137-25
Figure Lengend Snippet: Representative example of Candida biofilm formation on the Calgary Biofilm Device and plates used for determining MBC, MBEC, and MBDC. At the top, a schematic representation of biofilm formation on the MBEC Assay device is shown. Biofilm formation is observed as a yellow layer on each peg, while white pegs correspond to negative controls. At the bottom, in the challenge plate, released biofilm cells appear turbid in wells with growth, whereas translucent wells indicate no growth. The spot assay shows fungal growth on Sabouraud Dextrose Agar (SDA) after 24 hours of inoculation (5 µL from each well of the recovery plate). Within the black rectangles, hydroxyapatite powder is visible in the agar; this should not be confused with yeast growth. Finally, the MBDC recovery plate with Alamar Blue reveals metabolic activity, with blue indicating metabolic inactivity and pink indicating metabolic activity.
Article Snippet: This commercially available in vitro system, known as the MBEC Assay System (Innovotech, Canada), is widely used for biofilm-related studies ( , ).
Techniques: Spot Test, Activity Assay